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Sanger Sequencing for Microbial Identification

It includes practical experience in using Sanger sequencing for the identification of microorganisms, whether bacteria or fungi, and provides insight into how this sequencing method remains a gold standard in the field of infectious diseases.

Program Outline & Learning Objectives

  • DNA quantification and dilution of PCR products 
  • Purify PCR product  
  • Conduct cycle PCR  
  • Purify cycle PCR product  
  • Load the SeqStudio Flex Genetic Analyzer with the purified product  
  • Bioinformatic analysis: Trimming, alignment, BLAST  
  • Present your findings on why Sanger sequencing remains the gold standard in sequencing, recognized for its exceptional accuracy and reliability, to a diverse audience to enhance your science communication skills 

*prior knowledge and hands-on experiences in molecular biology required

Duration: 4 Days
Location: CHRF Headquarters (SEL Huq Skypark 23/2 Khilji Road Block-B, Mohammadpur Dhaka-1207)
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Program Fee

Sanger Sequencing for Microbial Identification Training (4 days): 10,000 BDT + 15% VAT applicable